Examinando por Autor "Ceccoli, Romina Denis"
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Ítem Acceso Abierto Flavoprotein monooxygenases for oxidative biocatalysis: recombinant expression in microbial hosts and applications(Frontiers Media, 2014-02-06) Ceccoli, Romina Denis; Bianchi, Dario A.; Rial, Daniela V.External flavoprotein monooxygenases comprise a group of flavin-dependent oxidoreductases that catalyze the insertion of one atom of molecular oxygen into an organic substrate and the second atom is reduced to water. These enzymes are involved in a great number of metabolic pathways both in prokaryotes and eukaryotes. Flavoprotein monooxygenases have attracted the attention of researchers for several decades and the advent of recombinant DNA technology caused a great progress in the field. These enzymes are subjected to detailed biochemical and structural characterization and some of them are also regarded as appealing oxidative biocatalysts for the production of fine chemicals and valuable intermediates toward active pharmaceutical ingredients due to their high chemo-, stereo-, and regioselectivity. Here, we review the most representative reactions catalyzed both in vivo and in vitro by prototype flavoprotein monooxygenases, highlighting the strategies employed to produce them recombinantly, to enhance the yield of soluble proteins, and to improve cofactor regeneration in order to obtain versatile biocatalysts. Although we describe the most outstanding features of flavoprotein monooxygenases, we mainly focus on enzymes that were cloned, expressed and used for biocatalysis during the last years.Ítem Acceso Abierto Visibilización del rol de las mujeres en la ciencia. Actividades para la reflexión(Universidad Nacional de La Plata, 2024-09-02) Di Capua, Cecilia Beatriz; Bortolotti, Ana; Ceccoli, Romina Denis; Campero, María Belén; https://orcid.org/0000-0002-8982-3489; https://orcid.org/0000-0002-7442-8005; https://orcid.org/0000-0002-2409-8482; https://orcid.org/0000-0001-8867-9836La conmemoración del Día Internacional de la Mujer y la Niña en la Ciencia tiene como propósito mejorar el acceso de las niñas a las ciencias y visibilizar el papel de las mujeres en la comunidad científica. En línea con las actividades que para esta fecha se realizan todos los años, en este artículo se abordan las acciones que en 2023 se llevaron a cabo en la Facultad de Ciencias Bioquímicas y Farmacéuticas de la Universidad Nacional de Rosario (exposiciones gráficas, conversatorios y experiencias científicas) con el objetivo de resaltar la importancia de integrar la perspectiva de género en la educación universitaria y de promover la equidad en el ámbito científico.