Examinando por Autor "Bauman, Carlos"
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Ítem Acceso Abierto Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway(Nature, 2017-01-30) Donato, Verónica; Rodríguez Ayala, Facundo; Cogliati, Sebastián; Bauman, Carlos; Costa, Juan Gabriel; Leñini, Cecilia; Grau, Roberto RicardoÍtem Acceso Abierto Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity(Shared Science Publishers, 2017-03-16) Rodríguez Ayala, Facundo; Bauman, Carlos; Cogliati, Sebastián; Leñini, Cecilia; Bartolini, Marco; Grau, Roberto RicardoÍtem Acceso Abierto Transcriptional regulation of adhesive properties of Bacillus subtilis to extracellular matrix proteins through the fibronectin-binding protein YloA(John Wiley & Sons Ltd, 2017-03-27) Rodríguez Ayala, Facundo; Bauman, Carlos; Bartolini, Marco; Saball, Ester; Salvarrey, Marcela; Leñini, Cecilia; Cogliati, Sebastián; Strauch, Mark; Grau, Roberto Ricardo; http://orcid.org/0000-0002-6496-3696Bacterial adherence to extracellular matrix proteins (ECMp) plays important roles during host–pathogen interaction, however its genetic regulation remains poorly understood. yloA of the model bacterium Bacillus subtilis shows high homology to genes encoding fibronectin-binding proteins of Gram-positive pathogens. Here, we characterized the regulatory network of YloA-dependent adhesive properties of the probiotic B. subtilis natto (Bsn). YloA-proficient, but not YloA-deficient, Bsn specifically bound to ECMp in a concentration-dependent manner and were proficient in biofilm formation. yloA expression showed a continuous increase in activity during the growth phase and decreased during the stationary phase. The transcription factors AbrB and DegU downregulated yloA expression during the logarithmic and stationary growth phases respectively. Analysis of the yloA promoter region revealed the presence of AT-rich direct and inverted repeats previously reported to function as DegU-recognized binding sites. In spo0A cells, yloA expression was completely turned off because of upregulation of AbrB throughout growth. Accordingly, DNase I footprinting analysis confirmed that AbrB bound to the promoter region of yloA. Interestingly, Bsn bound fibronectin with higher affinity, lower Kd, than several bacterial pathogens and competitively excluded them from binding to immobilized-fibronectin, a finding that might be important for the anti-infective properties of B. subtilis and its relatives.Ítem Acceso Abierto Two cases of type 2 diabetes mellitus successfully treated with probiotics(Wiley, 2020-12-21) Cardinali, Néstor; Bauman, Carlos; Rodríguez Ayala, Facundo; Grau, Roberto Ricardo; https://orcid.org/0000-0002-6496-3696The gut microbiota, and particularly probiotic bacteria, has emerged as a promising and novel intervention to fight the looming worldwide diabetes epidemic when combined with the appropriate medication. Herein, we report two cases of patient with type 2 diabetes refractory to conventional therapy that showed notable improvement after probiotic intervention.