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Transcriptional and metabolic profiling of potato plants expressing a plastid-targeted electron shuttle reveal modulation of genes associated to drought tolerance by chloroplast redox poise

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dc.citation.titleInternational Journal of Molecular Sciences
dc.citation.volume21
dc.contributor.orcidhttps://orcid.org/0000-0003-1739-4424
dc.contributor.orcidhttps://orcid.org/0000-0001-6549-6316
dc.contributor.orcidhttps://orcid.org/0000-0001-8236-7647
dc.contributor.orcidhttps://orcid.org/0000-0002-9185-6255
dc.creatorPierella Karlusich, Juan J.
dc.creatorArce, Rocío C.
dc.creatorShahinnia, Fahimeh
dc.creatorSonnewald, Sophia
dc.creatorSonnewald, Uwe
dc.creatorZurbriggen, Matias D.
dc.creatorHajirezaei, Mohammad-Reza
dc.creatorCarrillo, Néstor
dc.date.accessioned2024-06-04T14:13:06Z
dc.date.available2024-06-04T14:13:06Z
dc.date.issued2020-09-29
dc.description.abstractWater limitation represents the main environmental constraint affecting crop yield worldwide. Photosynthesis is a primary drought target, resulting in over-reduction of the photosynthetic electron transport chain and increased production of reactive oxygen species in plastids. Manipulation of chloroplast electron distribution by introducing alternative electron transport sinks has been shown to increase plant tolerance to multiple environmental challenges including hydric stress, suggesting that a similar strategy could be used to improve drought tolerance in crops. We show herein that the expression of the cyanobacterial electron shuttle flavodoxin in potato chloroplasts protected photosynthetic activities even at a pre-symptomatic stage of drought. Transcriptional and metabolic profiling revealed an attenuated response to the adverse condition in flavodoxin-expressing plants, correlating with their increased stress tolerance. Interestingly, 5–6% of leaf-expressed genes were affected by flavodoxin in the absence of drought, representing pathways modulated by chloroplast redox status during normal growth. About 300 of these genes potentially contribute to stress acclimation as their modulation by flavodoxin proceeds in the same direction as their drought response in wild-type plants. Tuber yield losses under chronic water limitation were mitigated in flavodoxin-expressing plants, indicating that the flavoprotein has the potential to improve major agronomic traits in potato.
dc.description.filFil: Pierella Karlusich, Juan J. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Arce, Rocío C. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Shahinnia, Fahimeh. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.
dc.description.filFil: Sonnewald, Sophia. Friedrich-Alexander-University Erlangen-Nurenberg. Division of Biochemistry, Department of Biology; Germany.
dc.description.filFil: Sonnewald, Uwe. Friedrich-Alexander-University Erlangen-Nurenberg. Division of Biochemistry, Department of Biology; Germany.
dc.description.filFil: Zurbriggen, Matias D. University of Düsseldorf. Institute of Synthetic Biology and CEPLAS; Germany.
dc.description.filFil: Hajirezaei, Mohammad-Reza. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.
dc.description.filFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2015-3828
dc.description.sponsorshipBunge-Born Foundation
dc.description.sponsorshipDeutscher Akademischer Austauschdienst (DAAD)
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO)
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG): CEPLAS–EXC– 2048/1–Project ID 390686111
dc.description.sponsorshipLeibniz institute of Plant Genetics and Crop Plant Research (IPK)
dc.format.extent1-22
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/2133/27170
dc.language.isoen
dc.publisherMDPI
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/21/19/7199
dc.relation.publisherversionhttps://doi.org/10.3390/ijms21197199
dc.rightsopenAccess
dc.rights.holderPierella Karlusich, Juan J.
dc.rights.holderArce, Rocío C.
dc.rights.holderShahinnia, Fahimeh
dc.rights.holderSonnewald, Sophia
dc.rights.holderSonnewald, Uwe
dc.rights.holderZurbriggen, Matias D.
dc.rights.holderHajirezaei, Mohammad-Reza
dc.rights.holderCarrillo, Néstor
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDrought
dc.subjectStress responses
dc.subjectPhotosynthesis
dc.subjectChloroplast redox status
dc.subjectFlavodoxin
dc.subjectTranscriptomics
dc.subjectMetabolomics
dc.titleTranscriptional and metabolic profiling of potato plants expressing a plastid-targeted electron shuttle reveal modulation of genes associated to drought tolerance by chloroplast redox poise
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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