Recombinant protein expression in Escherichia coli: advances and challenges
dc.citation.title | Frontiers in Microbiology | |
dc.citation.volume | 1-17 | |
dc.creator | Rosano, Germán L. | |
dc.creator | Ceccarelli, Eduardo Augusto | |
dc.date.accessioned | 2024-03-18T14:08:20Z | |
dc.date.available | 2024-03-18T14:08:20Z | |
dc.date.issued | 2014-04-17 | |
dc.description.abstract | Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field. | |
dc.description.fil | Fil: Rosano, Germán L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Rosano, Germán L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. | |
dc.description.fil | Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Ceccarelli, Eduardo Augusto. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. | |
dc.identifier.issn | 1664-302X | |
dc.identifier.uri | https://hdl.handle.net/2133/26795 | |
dc.language.iso | en | |
dc.publisher | Frontiers Media | |
dc.rights | openAccess | |
dc.rights.holder | Rosano, Germán L. | |
dc.rights.holder | Ceccarelli, Eduardo Augusto | |
dc.rights.holder | Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas | |
dc.rights.text | Attribution 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Recombinant protein expression | |
dc.subject | Escherichia coli | |
dc.subject | Expression plasmid | |
dc.subject | Inclusion bodies | |
dc.subject | Affinity tags | |
dc.subject | E. coli expression strains | |
dc.title | Recombinant protein expression in Escherichia coli: advances and challenges | |
dc.type | articulo | |
dc.type.collection | articulo | |
dc.type.version | publishedVersion |
Archivos
Bloque original
1 - 1 de 1
Cargando...
- Nombre:
- Recombinant protein expression in Escherichia coli advances and challenges.pdf
- Tamaño:
- 764.58 KB
- Formato:
- Adobe Portable Document Format
Bloque de licencias
1 - 1 de 1
- Nombre:
- license.txt
- Tamaño:
- 3.87 KB
- Formato:
- Item-specific license agreed upon to submission
- Descripción: