Synthesis of a deuterated standard for the quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
| dc.citation.title | Journal of Visualized Experiments | es |
| dc.citation.volume | 151 | es |
| dc.creator | Fernández de Luco, Julia | |
| dc.creator | Prez, Gastón M. | |
| dc.creator | Hernández Cravero, Bruno | |
| dc.creator | De Mendoza, Diego | |
| dc.creator | Labadie, Guillermo Roberto | |
| dc.date.accessioned | 2020-05-28T15:28:36Z | |
| dc.date.available | 2020-05-28T15:28:36Z | |
| dc.date.issued | 2019-09-21 | |
| dc.description | This work presents a method to prepare an analytical standard to analyze 2-arachidonoyl glycerol (2-AG) qualitatively and quantitatively by liquid chromatography-electrospray Ionization-tandem mass spectrometry (LC-ESI-MS/MS). Endocannabinoids are conserved lipid mediators that regulate multiple biological processes in a variety of organisms. In C. elegans, 2-AG has been found to possess different roles, including modulation of dauer formation and cholesterol metabolism. This report describes a method to overcome the difficulties associated with the costs and stability of deuterated standards required for 2-AG quantification. The procedure for the synthesis of the standard is simple and can be performed in any laboratory, without the need for organic synthesis expertise or special equipment. In addition, a modification of Folch's method to extract the deuterated standard from C. elegans culture is described. Finally, a quantitative and analytic method to detect 2-AG using the stable isotopically labeled analog 1-AG-d5 is described, which provides reliable results in a fast-chromatographic run. The procedure is useful for studying the multiple roles of 2-AG in C. elegans while also being applicable to other studies of metabolites in different organisms. | es |
| dc.description.fil | Fil: Fernández de Luco, Julia. Universidad Nacional del Rosario. Facultad de Ciencias Bioquímicas y Farmaceúticas. Instituto de Química Rosario (IQUIR-CONICET); Argentina. | es |
| dc.description.fil | Fil: Prez, Gastón M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Fisiología Microbiana (IBR-CONICET); Argentina. | es |
| dc.description.fil | Fil: Hernández Cravero, Bruno. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Fisiología Microbiana (IBR-CONICET); Argentina. | es |
| dc.description.fil | Fil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Fisiología Microbiana (IBR-CONICET); Argentina. | es |
| dc.description.fil | Fil: Labadie, Guillermo Roberto. Universidad Nacional del Rosario. Facultad de Ciencias Bioquímicas y Farmaceúticas. Instituto de Química Rosario (IQUIR-CONICET); Argentina. | es |
| dc.description.sponsorship | Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina): PICT 2014-3693 | es |
| dc.format | application/pdf | |
| dc.format.extent | ||
| dc.identifier.issn | 1940-087X | es |
| dc.identifier.uri | http://hdl.handle.net/2133/18279 | |
| dc.language.iso | eng | es |
| dc.publisher | JoVE | es |
| dc.relation.publisherversion | https://dx.doi.org/10.3791/59882 | es |
| dc.relation.publisherversion | https://www.jove.com/video/59882/synthesis-deuterated-standard-for-quantification-2 | es |
| dc.rights | embargoedAccess | es |
| dc.rights.holder | Fernández de Luco, Julia | es |
| dc.rights.holder | Prez, Gastón M. | es |
| dc.rights.holder | Hernández Cravero, Bruno | es |
| dc.rights.holder | De Mendoza, Diego | es |
| dc.rights.holder | Labadie, Guillermo Roberto | es |
| dc.rights.holder | The Journal of Visualized Experiments “JoVE” | es |
| dc.rights.holder | Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas | es |
| dc.rights.text | Atribución-NoComercial-SinDerivadas 3.0 No portada (CC BY-NC-ND 3.0) | es |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/deed.es | * |
| dc.subject | Endocannabinoids | es |
| dc.subject | C. elegans | es |
| dc.subject | Synthesis | es |
| dc.subject | Deuterated analogs | es |
| dc.subject | 2-AG | es |
| dc.subject | Dauer | es |
| dc.subject | MAGs | es |
| dc.subject | HPLC-MS/MS | en |
| dc.subject | Isotopic dilution | en |
| dc.subject | Quantification | en |
| dc.title | Synthesis of a deuterated standard for the quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans | es |
| dc.type | article | |
| dc.type | artículo | |
| dc.type | acceptedVersion | |
| dc.type.collection | articulo | |
| dc.type.version | acceptedVersion | es |