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The assembly of bacteria living in natural environments shapes neuronal integrity and behavioral outputs in Caenorhabditis elegans

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dc.citation.titlemBio
dc.citation.volume1-15
dc.citation.volume14
dc.contributor.orcidhttps://orcid.org/0000-0002-6168-7286
dc.creatorUrquiza Zurich, Sebastian
dc.creatorGarcia Angulo, Victor Antonio
dc.creatorBurdisso, Paula
dc.creatorPalominos, M. Fernanda
dc.creatorFernandez Hubeid, Lucia
dc.creatorHarcha, Paloma A.
dc.creatorCastillo, Juan P.
dc.creatorCalixto, Andrea
dc.date.accessioned2024-04-22T12:09:37Z
dc.date.available2024-04-22T12:09:37Z
dc.date.issued2023-04-25
dc.description.abstractBacterivore nematodes are the most abundant animals in the biosphere, largely contributing to global biogeochemistry. Thus, the effects of environmental microbes on the nematodes’ life-history traits are likely to contribute to the general health of the biosphere. Caenorhabditis elegans is an excellent model to study the behavioral and physiological outputs of microbial diets. However, the effects of complex natural bacterial assemblies have only recently been reported, as most studies have been carried out with monoxenic cultures of laboratory-reared bacteria. Here, we quantified the physiological, phenotypic, and behavioral traits of C. elegans feeding on two bacteria that were coisolated with wild nematodes from a soil sample. These bacteria were identified as a putative novel species of Stenotrophomonas named Stenotrophomonas sp. strain Iso1 and a strain of Bacillus pumilus designated Iso2. The distinctive behaviors and developmental patterns observed in animals fed with individual isolates changed when bacteria were mixed. We studied in more depth the degeneration rate of the touch circuit of C. elegans and show that B. pumilus alone is protective, while the mix with Stenotrophomonas sp. is degenerative. The analysis of the metabolite contents of each isolate and their combination identified NAD1 as being potentially neuroprotective. In vivo supplementation shows that NAD1 restores neuroprotection to the mixes and also to individual nonprotective bacteria. Our results highlight the distinctive physiological effects of bacteria resembling native diets in a multicomponent scenario rather than using single isolates on nematodes.
dc.description.filFil: Urquiza Zurich, Sebastian. Universidad de Valparaíso. Facultad de Ciencias. Centro Interdisciplinario de Neurociencia de Valparaíso; Chile.
dc.description.filFil: Garcia Angulo, Victor Antonio. Universidad de Chile. Facultad de Medicina. Instituto de Ciencias Biomédicas; Chile.
dc.description.filFil: Burdisso, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Burdisso, Paula. Plataforma Argentina de Biología Estructural y Metabolómica (PLABEM); Argentina.
dc.description.filFil: Palominos, M. Fernanda. Universidad de Valparaíso. Facultad de Ciencias. Centro Interdisciplinario de Neurociencia de Valparaíso; Chile.
dc.description.filFil: Fernandez Hubeid, Lucia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.
dc.description.filFil: Fernandez Hubeid, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Farmacología Experimental (CONICET-IFEC); Argentina.
dc.description.filFil: Harcha, Paloma A. Universidad de Valparaíso. Facultad de Ciencias. Centro Interdisciplinario de Neurociencia de Valparaíso; Chile.
dc.description.filFil: Castillo, Juan P. Universidad de Valparaíso. Facultad de Ciencias. Centro Interdisciplinario de Neurociencia de Valparaíso; Chile.
dc.description.filFil: Calixto, Andrea. Universidad de Valparaíso. Facultad de Ciencias. Centro Interdisciplinario de Neurociencia de Valparaíso; Chile.
dc.description.sponsorshipFondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT): 1220650
dc.description.sponsorshipMillennium Scientific Initiative: ICN09-022
dc.description.sponsorshipProyecto Apoyo Redes Formación de Centros: REDES180138, REDES190089
dc.identifier.issn2161-2129
dc.identifier.urihttps://hdl.handle.net/2133/26917
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.relation.publisherversionhttps://doi.org/10.1128/mbio.03402-22
dc.relation.publisherversionhttps://journals.asm.org/doi/10.1128/mbio.03402-22
dc.rightsopenAccess
dc.rights.holderUrquiza Zurich, Sebastian
dc.rights.holderGarcia Angulo, Victor Antonio
dc.rights.holderBurdisso, Paula
dc.rights.holderPalominos, M. Fernanda
dc.rights.holderFernandez Hubeid, Lucia
dc.rights.holderHarcha, Paloma A.
dc.rights.holderCastillo, Juan P.
dc.rights.holderCalixto, Andrea
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLife-history traits
dc.subjectMetabolomics
dc.subjectNematode
dc.subjectNeurodegeneration
dc.subjectWild
dc.subjectBacteria
dc.titleThe assembly of bacteria living in natural environments shapes neuronal integrity and behavioral outputs in Caenorhabditis elegans
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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