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Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.

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dc.citation.titlePLOS ONE
dc.citation.volume12(4)
dc.creatorChouhy, Diego
dc.creatorBolatti, Elisa María
dc.creatorSeme, Katja
dc.creatorPoljak, Mario
dc.creatorGiri, Adriana Angélica
dc.creatorŠterbenc, Anja
dc.creatorHošnjak, Lea
dc.creatorOštrbenk, Anja
dc.creatorKocjan, Boštjan J.
dc.creatorLuzar, Boštjan
dc.date.accessioned2017-11-25T02:06:34Z
dc.date.available2017-11-25T02:06:34Z
dc.date.issued2017
dc.descriptionHPV204 is the only newly identified Mupapillomavirus (Mu-PV) type in more than a decade. To comprehensively characterize HPV204, we performed a detailed molecular analysis of the viral genome and evaluated its clinical relevance in comparison to the other Mu-PVs, HPV1 and HPV63. The 7,227-bp long genome of HPV204 exhibits typical genomic organization of Mu-PVs with eight open reading frames (ORFs) (E6, E7, E1, E2, E8, E4, L2, and L1). We developed three type-specific quantitative real-time PCRs and used them to test a representative collection (n = 1,006) of various HPV-associated benign and malignant neoplasms, as well as samples of clinically normal cutaneous, mucosal, and mucocutaneous origins. HPV204, HPV1, and HPV63 were detected in 1.1%, 2.7%, and 1.9% of samples tested, respectively, and were present in skin and mucosa, suggesting dual tissue tropism of all Mu-PVs. To evaluate the etiological role of Mu-PVs in the development of HPV-associated neoplasms, Mu-PV viral loads per single cell were estimated. HPV1 and HPV63 were present in high viral copy numbers in 3/43 and 1/43 cutaneous warts, respectively, and were identified as the most likely causative agents of these warts. HPV204 viral load was extremely low in a single HPV204-positive cutaneous wart (7.4 × 10−7 viral copies/cell). Hence, etiological association between HPV204 and the development of cutaneous warts could not be established. To the best of our knowledge, this is the first study to evaluate the genetic variability of Mu-PVs by sequencing complete LCR genomic regions of HPV204, HPV1, and HPV63. We detected several nucleotide substitutions and deletions within the LCR genomic regions of Mu-PVs and identified two genetic variants of HPV204 and HPV63 and five genetic variants of HPV1.es
dc.description.filFil: Chouhy, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas. Área de Virología; Argentina
dc.description.filFil: Šterbenc, Anja. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.filFil: Hošnjak, Lea. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.filFil: Bolatti, Elisa María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas. Área de Virología; Argentina
dc.description.filFil: Oštrbenk, Anja. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.filFil: Seme, Katja. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.filFil: Kocjan, Boštjan J. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.filFil: Luzar, Boštjan. University of Ljubljana. Faculty of Medicine. Institute of Pathology; Slovenia
dc.description.filFil: Giri, Adriana Angélica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas. Área de Virología; Argentina
dc.description.filFil: Poljak, Mario. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.sponsorshipSlovenian Research Agency (ARRS), which funded AŠ through the Young Researcher Training Programes
dc.description.sponsorshipSlovenian-Argentinian Bilateral Project BI-AR-15-17-005es
dc.description.sponsorshipCoheaHr Project (European Commission FP7 Program, grant number: 603019)es
dc.formatapplication/pdf
dc.identifier.other1932-6203
dc.identifier.urihttp://hdl.handle.net/2133/9406
dc.language.isoenges
dc.publisherPLOSes
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175892es
dc.rightsopenAccesses
dc.rights.holderPLOSes
dc.rights.holderŠterbenc, Anjaes
dc.rights.holderHošnjak, Leaes
dc.rights.holderChouhy, Diegoes
dc.rights.holderBolatti, Elisa Maríaes
dc.rights.holderOštrbenk, Anjaes
dc.rights.holderSeme, Katjaes
dc.rights.holderKocjan, Boštjan J.es
dc.rights.holderLuzar, Boštjanes
dc.rights.holderGiri, Adriana Angélicaes
dc.rights.holderPoljak, Marioes
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectHPV204es
dc.subjectHPV1es
dc.subjectHPV63es
dc.subjectGenus Mu-PVes
dc.subjectMolecular characterizationes
dc.titleMolecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.es

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