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Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves

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dc.citation.titleFrontiers in Plant Sciencees
dc.citation.volume9es
dc.creatorMayta, Martín L.
dc.creatorLodeyro, Anabella F.
dc.creatorGuiamet, Juan J.
dc.creatorTognetti, Vanesa B.
dc.creatorMelzer, Michael
dc.creatorHajirezaei, Mohammad-Reza
dc.creatorCarrillo, Néstor
dc.date.accessioned2020-08-21T15:52:06Z
dc.date.available2020-08-21T15:52:06Z
dc.date.issued2018-07-17
dc.descriptionLeaf senescence is a concerted physiological process involving controlled degradation of cellular structures and reallocation of breakdown products to other plant organs. It is accompanied by increased production of reactive oxygen species (ROS) that are proposed to signal cell death, although both the origin and the precise role of ROS in the execution of this developmental program are still poorly understood. To investigate the contribution of chloroplast-associated ROS to natural leaf senescence, we used tobacco plants expressing a plastid-targeted flavodoxin, an electron shuttle flavoprotein present in prokaryotes and algae. When expressed in plants, flavodoxin specifically prevents ROS formation in chloroplasts during stress situations. Senescence symptoms were significantly mitigated in these transformants, with decreased accumulation of chloroplastic ROS and differential preservation of chlorophylls, carotenoids, protein contents, cell and chloroplast structures, membrane integrity and cell viability. Flavodoxin also improved maintenance of chlorophyll-protein complexes, photosynthetic electron flow, CO2 assimilation, central metabolic routes and levels of bioactive cytokinins and auxins in aging leaves. Delayed induction of senescence-associated genes indicates that the entire genetic program of senescence was affected by flavodoxin. The results suggest that ROS generated in chloroplasts are involved in the regulation of natural leaf senescence.es
dc.description.filFil: Mayta, Martín L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Lodeyro, Anabella F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Guiamet, Juan J. Universidad Nacional de La Plata. Instituto de Fisiología Vegetal (INFIVE-CONICET); Argentina.es
dc.description.filFil: Tognetti, Vanesa B. Masaryk University. Central European Institute of Technology. Mendel Centre for Plant Genomics and Proteomics; Czechia.es
dc.description.filFil: Melzer, Michael. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.es
dc.description.filFil: Hajirezaei, Mohammad-Reza. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.es
dc.description.filFil: Néstor Carrillo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT-2014-2496es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT-2015-3828es
dc.description.sponsorshipFundación Josefina Prats (Argentina)es
dc.description.sponsorshipLeibniz Institute of Plant Genetics and Crop Plant Researches
dc.description.sponsorshipCzech Science Foundation: GA15-16520Ses
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)es
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO)es
dc.description.sponsorshipUniversidad Nacional de Rosarioes
dc.description.sponsorshipUniversidad Nacional de La Plataes
dc.formatapplication/pdf
dc.format.extent1039es
dc.identifier.issn1664-462Xes
dc.identifier.urihttp://hdl.handle.net/2133/18694
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fpls.2018.01039/full#h9es
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2018.01039es
dc.rightsopenAccesses
dc.rights.holderMayta, Martín L.es
dc.rights.holderLodeyro, Anabella F.es
dc.rights.holderGuiamet, Juan J.es
dc.rights.holderTognetti, Vanesa B.es
dc.rights.holderMelzer, Michaeles
dc.rights.holderHajirezaei, Mohammad-Rezaes
dc.rights.holderNéstor Carrilloes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectSenescencees
dc.subjectChloroplastses
dc.subjectReactive oxygen specieses
dc.subjectRedox poisees
dc.subjectFlavodoxines
dc.subjectTransgenic plantses
dc.subjectTobaccoes
dc.titleExpression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaveses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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