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Efficiency and precision of microRNA biogenesis modes in plants

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dc.citation.titleNucleic Acids Researches
dc.citation.volume46(20)es
dc.creatorMoro, Belén
dc.creatorChorostecki, Uciel Pablo
dc.creatorArikit, Siwaret
dc.creatorSuárez, Irina Paula
dc.creatorHöbartner, Claudia
dc.creatorRasia, Rodolfo M.
dc.creatorMeyers, Blake C.
dc.creatorPalatnik, Javier F.
dc.date.accessioned2021-03-04T19:26:04Z
dc.date.available2021-03-04T19:26:04Z
dc.date.issued2018-10-05
dc.descriptionMany evolutionarily conserved microRNAs (miRNAs) in plants regulate transcription factors with key functions in development. Hence, mutations in the core components of the miRNA biogenesis machinery cause strong growth defects. An essential aspect of miRNA biogenesis is the precise excision of the small RNA from its precursor. In plants, miRNA precursors are largely variable in size and shape and can be processed by different modes. Here, we optimized an approach to detect processing intermediates during miRNA biogenesis. We characterized a miRNA whose processing is triggered by a terminal branched loop. Plant miRNA processing can be initiated by internal bubbles, small terminal loops or branched loops followed by dsRNA segments of 15–17 bp. Interestingly, precision and efficiency vary with the processing modes. Despite the various potential structural determinants present in a single a miRNA precursor, DCL1 is mostly guided by a predominant structural region in each precursor in wildtype plants. However, our studies in fiery1, hyl1 and se mutants revealed the existence of cleavage signatures consistent with the recognition of alternative processing determinants. The results provide a general view of the mechanisms underlying the specificity of miRNA biogenesis in plants.es
dc.descriptionThis article has been accepted for publication in Nucleic Acids Research Published by Oxford University Press.
dc.description.filFil: Moro, Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Moro, Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Chorostecki, Uciel Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Chorostecki, Uciel Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Arikit, Siwaret. Kasetsart University. Department of Agronomy, Kamphaeng Saen and Rice Science Center; Thailand.es
dc.description.filFil: Suárez, Irina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Höbartner, Claudia. Universität Würzburg. Institut für Organische Chemie; Deutchland.es
dc.description.filFil: Rasia, Rodolfo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Rasia, Rodolfo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Meyers, Blake C. Donald Danforth Plant Science Center; United States.es
dc.description.filFil: Meyers, Blake C. University of Missouri. Department of Plant Science; United States.es
dc.description.filFil: Palatnik, Javier F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Palatnik, Javier F. Universidad Nacional de Rosario. Centro de Estudios Interdisciplinarios (CEI); Argentina.es
dc.description.sponsorshipInternational Centre for Genetic Engineering and Biotechnology (ICGEB): CRP/ARG17-01 and PICT-2016–0761es
dc.description.sponsorshipUS National Science Foundation (IOS program): award #1339229es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET) – National Natural Science Foundation of China (NSFC) (Programa de Cooperación Bilateral): project 540/16es
dc.formatapplication/pdf
dc.format.extent10709–10723es
dc.identifier.issn1362-4962es
dc.identifier.urihttp://hdl.handle.net/2133/20015
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gky853es
dc.relation.publisherversionhttps://academic.oup.com/nar/article/46/20/10709/5115824es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderMoro, Belénes
dc.rights.holderChorostecki, Uciel Pabloes
dc.rights.holderArikit, Siwaretes
dc.rights.holderSuárez, Irina Paulaes
dc.rights.holderHöbartner, Claudiaes
dc.rights.holderRasia, Rodolfo M.es
dc.rights.holderMeyers, Blake C.es
dc.rights.holderPalatnik, Javier F.es
dc.rights.textAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectGene Expression Regulation, Plantes
dc.subjectTranscription, Genetices
dc.subjectBinding Siteses
dc.subjectTransgeneses
dc.subjectRNA-Binding Proteinses
dc.subjectRNA, Double-Strandedes
dc.titleEfficiency and precision of microRNA biogenesis modes in plantses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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