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Identification of Novel Thermosensors in Gram-Positive Pathogens

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dc.citation.titleFrontiers in Molecular Biosciences
dc.citation.volume7
dc.creatorFernández, Pilar
dc.creatorDíaz, Alejandra Raquel
dc.creatorRé, María Florencia
dc.creatorPorrini, Lucía
dc.creatorDe Mendoza, Diego
dc.creatorAlbanesi, Daniela
dc.creatorMansilla, María Cecilia
dc.date.accessioned2022-04-01T20:13:46Z
dc.date.available2022-04-01T20:13:46Z
dc.date.issued2020-11-26
dc.descriptionTemperature is a crucial variable that every living organism, from bacteria to humans, need to sense and respond to in order to adapt and survive. In particular, pathogenic bacteria exploit host-temperature sensing as a cue for triggering virulence gene expression. Here, we have identified and characterized two integral membrane thermosensor histidine kinases (HKs) from Gram-positive pathogens that exhibit high similarity to DesK, the extensively characterized cold sensor histidine kinase from Bacillus subtilis. Through in vivo experiments, we demonstrate that SA1313 from Staphylococcus aureus and BA5598 from Bacillus anthracis, which likely control the expression of putative ATP binding cassette (ABC) transporters, are regulated by environmental temperature. We show here that these HKs can phosphorylate the noncognate response regulator DesR, partner of DesK, both in vitro and in vivo, inducing in B. subtilis the expression of the des gene upon a cold shock. In addition, we report the characterization of another DesK homolog from B. subtilis, YvfT, also closely associated to an ABC transporter. Although YvfT phosphorylates DesR in vitro, this sensor kinase can only induce des expression in B. subtilis when overexpressed together with its cognate response regulator YvfU. This finding evidences a physiological mechanism to avoid cross talk with DesK after a temperature downshift. Finally, we present data suggesting that the HKs studied in this work appear to monitor different ranges of membrane lipid properties variations to mount adaptive responses upon cooling. Overall, our findings point out that bacteria have evolved sophisticated mechanisms to assure specificity in the response to environmental stimuli. These findings pave the way to understand thermosensing mediated by membrane proteins that could have important roles upon host invasion by bacterial pathogens.es
dc.description.filFil: Fernández, Pilar. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ré, María Florencia. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: De Mendoza, Diego. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Albanesi, Daniela. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Mansilla, María Cecilia. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Díaz, Alejandra Raquel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Centro de Recursos Naturales Renovables de la Zona Semi-árida (CERZOS-CONICET); Argentina.
dc.description.filFil: Porrini, Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Albanesi, Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Mansilla, María Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas
dc.description.sponsorshipUniversidad Nacional de Rosario: 1BIO367
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas: PUE-2016-0039
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PID-2013-0043
dc.description.sponsorshipUniversidad Nacional del Sur: PGI 24/ZB53
dc.formatapplication/pdf
dc.format.extent1-12
dc.identifier.urihttp://hdl.handle.net/2133/23310
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmolb.2020.592747/fulles
dc.relation.publisherversionhttps://doi.org/10.3389/fmolb.2020.592747
dc.rightsopenAccesses
dc.rights.holderFernández, Pilares
dc.rights.holderDíaz, Alejandra Raqueles
dc.rights.holderRé, María Florenciaes
dc.rights.holderPorrini, Lucíaes
dc.rights.holderDe Mendoza, Diegoes
dc.rights.holderAlbanesi, Danielaes
dc.rights.holderMansilla, María Ceciliaes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectThermosensores
dc.subjectGram Positive Pathogenes
dc.subjectABC Transporteres
dc.subjectTwo Component Systemes
dc.subjectSignallinges
dc.titleIdentification of Novel Thermosensors in Gram-Positive Pathogenses
dc.typepublishedVersion
dc.typeartículo
dc.typearticle
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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