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KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells

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dc.citation.titlePLOS ONEes
dc.citation.volume14(1)es
dc.creatorDomizi, Pablo
dc.creatorMalizia, Florencia
dc.creatorChazarreta-Cifre, Lorena
dc.creatorDiacovich, Lautaro
dc.creatorBanchio, Claudia
dc.date.accessioned2021-02-22T23:17:38Z
dc.date.available2021-02-22T23:17:38Z
dc.date.issued2019-01-10
dc.descriptionThe process of neuronal differentiation is associated with neurite elongation and membrane biogenesis, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. During neuroblast differentiation, the transcription of two genes involved in PtdCho biosynthesis are stimulated: Chka gene for choline kinase (CK) alpha isoform and Pcyt1a gene for CTP:phosphocholine cytidylyltransferase (CCT) alpha isoform. Here we show that CKα is essential for neuronal differentiation. In addition, we demonstrated that KDM2B regulates CKα expression and, as a consequence, neuronal differentiation. This factor is up-regulated in the course of the neuroblasts proliferative and undifferentiated state and down-regulated during differentiation induced by retinoic acid (RA). During proliferation, KDM2B binds to the Box2 located in the Chka promoter repressing its transcription. Interestingly, KDM2B knockdown enhances the levels of CKα expression in neuroblast cells and induces neuronal differentiation even in the absence of RA. These results suggest that KDM2B is required for the appropriate regulation of CKα during neuronal differentiation and to the maintaining of the undifferentiated stage of neuroblast cells.es
dc.descriptionPara citar este articulo: Domizi P, Malizia F, Chazarreta-Cifre L, Diacovich L, Banchio C (2019) KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells. PLoS ONE 14(1): e0210207. https://doi.org/10.1371/journal. pone.0210207
dc.description.filFil: Domizi, Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Malizia, Florencia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Chazarreta-Cifre, Lorena. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Diacovich, Lautaro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Banchio, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica (FONCYT): PICT2013es
dc.formatapplication/pdf
dc.format.extent1-15es
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/2133/19669
dc.language.isoenges
dc.publisherPublic Library of Science (PLOS)es
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0210207es
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0210207es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderDomizi, Pabloes
dc.rights.holderMalizia, Florenciaes
dc.rights.holderDiacovich, Lautaroes
dc.rights.holderBanchio, Claudiaes
dc.rights.holderChazarreta-Cifre, Lorenaes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectKDM2B Genees
dc.subjectCholine Kinase Expressiones
dc.subjectNeuronal Differentiationes
dc.subjectNeuroblastoma Cellses
dc.titleKDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cellses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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