3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
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Date
2018-06-07
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Public Library of Science (PLOS)
Abstract
Description
Citrus canker is a disease caused by the phytopathogen Xanthomonas citri subsp. citri (Xcc),
bacterium which is unable to survive out of the host for extended periods of time. Once established inside the plant, the pathogen must compete for resources and evade the defenses of
the host cell. However, a number of aspects of Xcc metabolic and nutritional state, during the
epiphytic stage and at different phases of infection, are poorly characterized. The 3-methylcrotonyl-CoA carboxylase complex (MCC) is an essential enzyme for the catabolism of the
branched-chain amino acid leucine, which prevents the accumulation of toxic intermediaries,
facilitates the generation of branched chain fatty acids and/or provides energy to the cell. The
MCC complexes belong to a group of acyl-CoA carboxylases (ACCase) enzymes dependent
of biotin. In this work, we have identified two ORFs (XAC0263 and XAC0264) encoding for
the Ī± and Ī² subunits of an acyl-CoA carboxylase complex from Xanthomonas and demonstrated that this enzyme has MCC activity both in vitro and in vivo. We also found that this
MCC complex is conserved in a group of pathogenic gram negative bacteria. The generation
and analysis of an Xcc mutant strain deficient in MCC showed less canker lesions in the interaction with the host plant, suggesting that the expression of these proteins is necessary for
Xcc fitness during infection.
Para citar este articulo: Tomassetti M, Garavaglia BS, Vranych CV, Gottig N, Ottado J, Gramajo H, et al. (2018) 3- methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection. PLoS ONE 13(6): e0198414. https://doi.org/10.1371/ journal.pone.0198414
Para citar este articulo: Tomassetti M, Garavaglia BS, Vranych CV, Gottig N, Ottado J, Gramajo H, et al. (2018) 3- methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection. PLoS ONE 13(6): e0198414. https://doi.org/10.1371/ journal.pone.0198414
Keywords
Xanthomonas citri subsp. citri, Citrus Canker, Methylcrotonyl Coenzyme A (CoA) carboxylase complex