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Implications of the expression of Enterococcus faecalis citrate fermentation genes during infection

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dc.citation.titlePLoS ONE
dc.contributor.orcidhttp://orcid.org/0000-0003-0508-260X
dc.contributor.orcidhttp://orcid.org/0000-0003-3945-2859
dc.contributor.otherLópez, Paloma: provide the pTLGR plasmid.
dc.contributor.otherRequena, Teresa: provide the pTLGR plasmid.
dc.contributor.otherChapo, Gustavo: provide the defibrinated blood.
dc.contributor.otherGonzález, Fabián: provide the defibrinated blood.
dc.creatorMartino, Gabriela Paula
dc.creatorPérez, Cristian E.
dc.creatorMagni, Christian
dc.creatorBlancato, Víctor Sebastián
dc.date.accessioned2024-06-03T21:06:28Z
dc.date.available2024-06-03T21:06:28Z
dc.date.issued2018-10-18
dc.description.abstractCitrate is an ubiquitous compound in nature. However, citrate fermentation is present only in a few pathogenic or nonpathogenic microorganisms. The citrate fermentation pathway includes a citrate transporter, a citrate lyase complex, an oxaloacetate decarboxylase and a regulatory system. Enterococcus faecalis is commonly present in the gastro-intestinal microbiota of warm-blooded animals and insect guts. These bacteria can also cause infection and disease in immunocompromised individuals. In the present study, we performed whole genome analysis in Enterococcus strains finding that the complete citrate pathway is present in all of the E. faecalis strains isolated from such diverse habitats as animals, hospitals, water, milk, plants, insects, cheese, etc. These results indicate the importance of this,metabolic preservation for persistence and growth of E. faecalis in different niches. We also analyzed the role of citrate metabolism in the E. faecalis pathogenicity. We found that an E. faecalis citrate fermentation-deficient strain was less pathogenic for Galleria mellonella larvae than the wild type. Furthermore, strains with deletions in the oxaloacetate decarboxylase subunits or in the α-acetolactate synthase resulted also less virulent than the wild type strain. We also observed that citrate promoters are induced in blood, urine and also in the hemolymph of G. mellonella. In addition, we showed that citrate fermentation allows E. faecalis to grow better in blood, urine and G. mellonella. The results presented here clearly indicate that citrate fermentation plays an important role in E. faecalis opportunistic pathogenic behavior.
dc.description.filFil: Martino, Gabriela Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Fisiología y Genética de Bacterias (IBR-CONICET); Argentina.
dc.description.filFil: Pérez, Cristian E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Fisiología y Genética de Bacterias (IBR-CONICET); Argentina.
dc.description.filFil: Magni, Christian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Fisiología y Genética de Bacterias (IBR-CONICET); Argentina.
dc.description.filFil: Magni, Christian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquimicas y Farmacéuticas. Laboratorio de Biotecnología e Inocuidad de los Alimentos; Argentina.
dc.description.filFil: Magni, Christian. Municipalidad de Baigorria; Argentina.
dc.description.filFil: Blancato, Víctor Sebastián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Fisiología y Genética de Bacterias (IBR-CONICET); Argentina.
dc.description.filFil: Blancato, Víctor Sebastián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Biotecnología e Inocuidad de los Alimentos; Argentina.
dc.description.filFil: Blancato, Víctor Sebastián. Municipalidad de Baigorria; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPyCT): PICT 2014 – 3482
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 11220150100855
dc.description.versionpeerreviewed
dc.format.extent1-18
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2133/27148
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0205787
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205787#ack
dc.rightsopenAccess
dc.rights.holderMartino, Gabriela Paula
dc.rights.holderPérez, Cristian E.
dc.rights.holderMagni, Christian
dc.rights.holderBlancato, Víctor Sebastián
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEnterococcus faecalis
dc.subjectBacterial proteins
dc.subjectCarboxy-lyases
dc.subjectCarrier proteins
dc.subjectGene expression regulation
dc.subjectGram-positive bacterial infections
dc.subjectGram-positive bacterial infections
dc.titleImplications of the expression of Enterococcus faecalis citrate fermentation genes during infection
dc.typearticulo
dc.type.versionpublishedVersion

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