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Alpha-tubulin acetylation in Trypanosoma cruzi: a dynamic instabilityof microtubules is required for replication and cell cycle progression

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dc.citation.titleFrontiers in Cellular and Infection Microbiology
dc.citation.volume11
dc.creatorAlonso, Victoria Lucía
dc.creatorCarloni, Mara Emilia
dc.creatorSilva Gonçalves, Camila
dc.creatorMartínez Peralta, Gonzálo
dc.creatorChesta, Maria Eugenia
dc.creatorPezza, Alejandro
dc.creatorTavernelli, Luis Emilio
dc.creatorMotta, María Cristina M.
dc.creatorSerra, Esteban Carlos
dc.date.accessioned2024-06-14T12:27:55Z
dc.date.available2024-06-14T12:27:55Z
dc.date.issued2021-03-11
dc.description.abstractTrypanosomatids have a cytoskeleton arrangement that is simpler than what is found in most eukaryotic cells. However, it is precisely organized and constituted by stable microtubules. Such microtubules compose the mitotic spindle during mitosis, the basal body, the flagellar axoneme and the subpellicular microtubules, which are connected to each other and also to the plasma membrane forming a helical arrangement along the central axis of the parasite cell body. Subpellicular, mitotic and axonemal microtubules are extensively acetylated in Trypanosoma cruzi. Acetylation on lysine (K) 40 of a-tubulin is conserved from lower eukaryotes to mammals and is associated with microtubule stability. It is also known that K40 acetylation occurs significantly on flagella, centrioles, cilia, basal body and the mitotic spindle in eukaryotes. Several tubulin posttranslational modifications, including acetylation of K40, have been cataloged in trypanosomatids, but the functional importance of these modifications for microtubule dynamics and parasite biology remains largely undefined. The primary tubulin acetyltransferase was recently identified in several eukaryotes as Mec-17/ATAT, a Gcn5-related N-acetyltransferase. Here, we report that T. cruzi ATAT acetylates a-tubulin in vivo and is capable of autoacetylation. TcATAT is located in the cytoskeleton and flagella of epimastigotes and colocalizes with acetylated a-tubulin in these structures. We have expressed TcATAT with an HA tag using the inducible vector pTcINDEX-GW in T. cruzi. Over-expression of TcATAT causes increased levels of the alpha tubulin acetylated species, induces morphological and ultrastructural defects, especially in the mitochondrion, and causes a halt in the cell cycle progression of epimastigotes, which is related to an impairment of the kinetoplast division. Finally, as a result of TcATAT over-expression we observed that
dc.description.filFil: Alonso, Victoria Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Biología y Bioquímica de Trypanosoma cruzi (IBR-CONICET); Argentina.
dc.description.filFil: Alonso, Victoria Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.filFil: Carloni, Mara Emilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.filFil: Silva Gonçalves, Camila. Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer; Brazil.
dc.description.filFil: Silva Gonçalves, Camila. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens; Brazil.
dc.description.filFil: Martínez Peralta, Gonzálo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Biología y Bioquímica de Trypanosoma cruzi (IBR-CONICET); Argentina.
dc.description.filFil: Martínez Peralta, Gonzálo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.filFil: Chesta, Maria Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina.
dc.description.filFil: Pezza, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Biología y Bioquímica de Trypanosoma cruzi (IBR-CONICET); Argentina.
dc.description.filFil: Tavernelli, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Biología y Bioquímica de Trypanosoma cruzi (IBR-CONICET); Argentina.
dc.description.filFil: Motta, Maria Cristina M. Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer; Brazil.
dc.description.filFil: Motta, María Cristina M. Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens; Brazil.
dc.description.filFil: Serra, Esteban Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Biología y Bioquímica de Trypanosoma cruzi (IBR-CONICET); Argentina.
dc.description.filFil: Serra, Esteban Carlos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT2017-1978
dc.description.sponsorshipUniversidad Nacional de Rosario: 1BIO490
dc.description.sponsorshipGlobal Challenges Research Fund: MR/P027989/1
dc.description.versionpeerreviewed
dc.format.extent1-17
dc.identifier.issn2235-2988
dc.identifier.urihttps://hdl.handle.net/2133/27307
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.642271/full
dc.relation.publisherversionhttps://doi.org/10.3389/fcimb.2021.642271
dc.rightsopenAccess
dc.rights.holderAlonso, Victoria Lucía
dc.rights.holderCarloni, Mara Emilia
dc.rights.holderSilva Gonçalves, Camila
dc.rights.holderMartínez Peralta, Gonzálo
dc.rights.holderChesta, Maria Eugenia
dc.rights.holderPezza, Alejandro
dc.rights.holderTavernelli, Luis Emilio
dc.rights.holderMotta, María Cristina M.
dc.rights.holderSerra, Esteban Carlos
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTubulin
dc.subjectCytoskeleton
dc.subjectFlagella
dc.subjectAcetylation
dc.subjectCell division
dc.titleAlpha-tubulin acetylation in Trypanosoma cruzi: a dynamic instabilityof microtubules is required for replication and cell cycle progression
dc.typearticulo
dc.type.versionpublishedVersion

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