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Revisiting the cell biology of the acyl-ACP:phosphate transacylase PlsX suggests that the phospholipid synthesis and cell division machineries are not coupled in Bacillus subtilis

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dc.citation.titleMolecular Microbiology
dc.citation.volume100
dc.creatorSastre, Diego Emiliano
dc.creatorBisson-Filho, Alexandre
dc.creatorDe Mendoza, Diego
dc.creatorGueiros-Filho, Frederico J.
dc.date.accessioned2024-07-26T12:38:23Z
dc.date.available2024-07-26T12:38:23Z
dc.date.issued2016-05-06
dc.description.abstractPlsX is a central enzyme of phospholipid synthesis in bacteria, converting acyl-ACP to acyl-phosphate on the pathway to phosphatidic acid formation. PlsX has received attention because it plays a key role in the coordination of fatty acid and phospholipid synthesis. Recently, PlsX was also suggested to coordinate membrane synthesis with cell division in Bacillus subtilis. Here, we have re-investigated the cell biology of PlsX and determined that the enzyme is uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well. Foci and homogenous patterns seem freely interconvertible but the prevalence of the uniform staining suggests that PlsX does not need to localize to specific sites to function correctly. We also investigated the relationship between PlsX and the divisome. In contrast to previous observations, PlsX's foci showed no obvious periodicity of localization and did not colocalize with the divisome. Furthermore, depletion of PlsX did not affect cell division if phospholipid synthesis is maintained by an alternative enzyme. These results suggest that coordination between division and membrane synthesis may not require physical or functional interactions between the divisome and phospholipid synthesis enzymes.
dc.description.filFil: Sastre, Diego Emiliano. Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica; Brasil.
dc.description.filFil: Bisson-Filho, Alexandre. Harvard University. Faculty of Arts and Sciences. FAS Center for Systems Biology; United States.
dc.description.filFil: Bisson-Filho, Alexandre. Harvard University. Department of Molecular and Cellular Biology (MCB); United States.
dc.description.filFil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Gueiros-Filho, Frederico J. Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica; Brasil.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES): grant 1169/2013
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP): postdoctoral fellowship 2014/13411-1
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCiências Sem Fronteiras: postdoctoral fellowship 206227/2014-0
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.versionpeerreviewed
dc.format.extent1-14
dc.identifier.e-issn1365-2958
dc.identifier.issn0950-382X
dc.identifier.urihttps://hdl.handle.net/2133/27473
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1111/mmi.13337
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/mmi.13337
dc.rightsopenAccess
dc.rights.holderSastre, Diego Emiliano
dc.rights.holderBisson-Filho, Alexandre
dc.rights.holderDe Mendoza, Diego
dc.rights.holderGueiros-Filho, Frederico J.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPlsX
dc.subjectBacillus subtilis
dc.subjectAcyl carrier protein
dc.subjectCell biology
dc.subjectPhospholipid synthesis
dc.subjectCell division
dc.titleRevisiting the cell biology of the acyl-ACP:phosphate transacylase PlsX suggests that the phospholipid synthesis and cell division machineries are not coupled in Bacillus subtilis
dc.typearticulo
dc.type.versionpublishedVersion

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