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The pleiotropic transcriptional regulator NlpR contributes to the modulation of nitrogen metabolism, lipogenesis and triacylglycerol accumulation in oleaginous rhodococci

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dc.citation.titleMolecular Microbiology
dc.citation.volume103
dc.creatorHernández, Martín Alejandro
dc.creatorLara, María Julia
dc.creatorGago, Gabriela
dc.creatorGramajo, Hugo Cesar
dc.creatorÁlvarez, Héctor Manuel
dc.date.accessioned2024-06-10T20:37:05Z
dc.date.available2024-06-10T20:37:05Z
dc.date.issued2016-11-25
dc.description.abstractThe regulatory mechanisms involved in lipogenesis and triacylglycerol (TAG) accumulation are largely unknown in oleaginous rhodococci. In this study a regulatory protein (here called NlpR: Nitrogen lipid Regulator), which contributes to the modulation of nitrogen metabolism, lipogenesis and triacylglycerol accumulation in oleaginous rhodococci was identified. Under nitrogen deprivation conditions, in which TAG accumulation is stimulated, the nlpR gene was significantly upregulated, whereas a significant decrease of its expression and TAG accumulation occurred when cerulenin was added. The nlpR disruption negatively affected the nitrate/nitrite reduction as well as lipid biosynthesis under nitrogen-limiting conditions. In contrast, its overexpression increased TAG production during cultivation of cells in nitrogen-rich media. A putative ‘NlpR-binding motif’ upstream of several genes related to nitrogen and lipid metabolisms was found. The nlpR disruption in RHA1 strain led to a reduced transcription of genes involved in nitrate/nitrite assimilation, as well as in fatty acid and TAG biosynthesis. Purified NlpR was able to bind to narK, nirD, fasI, plsC and atf3 promoter regions. It was suggested that NlpR acts as a pleiotropic transcriptional regulator by activating of nitrate/nitrite assimilation genes and others genes involved in fatty acid and TAG biosynthesis, in response to nitrogen deprivation.
dc.description.filFil: Hernández, Martín Alejandro. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales. Instituto de Biociencias de la Patagonia (INBIOP-CONICET); Argentina.
dc.description.filFil: Lara, María Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gago, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Álvarez, Héctor Manuel. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales. Instituto de Biociencias de la Patagonia (INBIOP-CONICET); Argentina.
dc.description.sponsorshipUniversidad Nacional de la Patagonia San Juan Bosco. Secretaría de Ciencia y Técnica
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): project PIP2015-2017 nro. 0529
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT2012-2031, PICT2013-0916
dc.description.versionpeerreviewed
dc.format.extent1-20
dc.identifier.e-issn1365-2958
dc.identifier.issn0950-382X
dc.identifier.urihttps://hdl.handle.net/2133/27295
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1111/mmi.13564
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/mmi.13564
dc.rightsopenAccess
dc.rights.holderHernández, Martín Alejandro
dc.rights.holderLara, María Julia
dc.rights.holderGago, Gabriela
dc.rights.holderGramajo, Hugo Cesar
dc.rights.holderÁlvarez, Héctor Manuel
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectTranscriptional regulation
dc.subjectRhodococcus
dc.subjectNitrogen lipid regulator
dc.subjectNlpR
dc.subjectBiosynthesis
dc.subjectMetabolism
dc.subjectNitrogen
dc.subjectLipogenesis
dc.subjectTriglycerides
dc.titleThe pleiotropic transcriptional regulator NlpR contributes to the modulation of nitrogen metabolism, lipogenesis and triacylglycerol accumulation in oleaginous rhodococci
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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