Molecular fingerprints to identify Candida species
dc.citation.title | BioMed Research International | |
dc.citation.volume | 1-11 | |
dc.citation.volume | 2013 | |
dc.creator | Spampinato, Claudia P. | |
dc.creator | Leonardi, Darío | |
dc.date.accessioned | 2024-04-18T17:36:55Z | |
dc.date.available | 2024-04-18T17:36:55Z | |
dc.date.issued | 2013-06-17 | |
dc.description.abstract | A wide range of molecular techniques have been developed for genotyping Candida species. Among them, multilocus sequence typing (MLST) and microsatellite length polymorphisms (MLP) analysis have recently emerged. MLST relies on DNA sequences of internal regions of various independent housekeeping genes, while MLP identifies microsatellite instability. Both methods generate unambiguous and highly reproducible data. Here, we review the results achieved by using these two techniques and also provide a brief overview of a new method based on high-resolution DNA melting (HRM). This method identifies sequence differences by subtle deviations in sample melting profiles in the presence of saturating fluorescent DNA binding dyes | |
dc.description.fil | Fil: Spampinato, Claudia P. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica; Argentina. | |
dc.description.fil | Fil: Spampinato, Claudia P. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos (CONICET-CEFOBI); Argentina. | |
dc.description.fil | Fil: Leonardi, Darío. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Tecnología Farmacéutica; Argentina. | |
dc.description.fil | Fil: Leonardi, Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Rosario (CONICET-IQUIR); Argentina | |
dc.description.sponsorship | Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 0458, PICT 2643 | |
dc.description.sponsorship | Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 0018 | |
dc.description.sponsorship | Universidad Nacional de Rosario (UNR): BIO 221, BIO 328 | |
dc.identifier.issn | 2314-6141 | |
dc.identifier.uri | https://hdl.handle.net/2133/26907 | |
dc.language.iso | en | |
dc.publisher | Hindawi | |
dc.relation.publisherversion | https://www.hindawi.com/journals/bmri/2013/923742/#copyright | |
dc.relation.publisherversion | https://doi.org/10.1155/2013/923742 | |
dc.rights | openAccess | |
dc.rights.holder | Spampinato, Claudia P. | |
dc.rights.holder | Leonardi, Darío | |
dc.rights.holder | Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas | |
dc.rights.text | Attribution 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Candida | |
dc.subject | Nucleic acid denaturation | |
dc.subject | Species specificity | |
dc.subject | Microsatellite repeats | |
dc.subject | Multilocus sequence typing | |
dc.title | Molecular fingerprints to identify Candida species | |
dc.type | articulo | |
dc.type.collection | articulo | |
dc.type.version | publishedVersion |