Endocannabinoid 2-arachidonoyl glycerol increases the transcription of daf-7 in ASI neurons
| dc.citation.title | microPublication Biology | |
| dc.creator | Galles, Celina | |
| dc.creator | Prez, Gastón M. | |
| dc.creator | De Mendoza, Diego | |
| dc.date.accessioned | 2024-04-24T19:23:47Z | |
| dc.date.available | 2024-04-24T19:23:47Z | |
| dc.date.issued | 2018-10-19 | |
| dc.description.abstract | This article investigates the effect of the endocannabinoid 2-arachidonoyl glycerol (2-AG) on the expression of the daf-7 gene in ASI neurons of nematodes. It demonstrates that daf-7 expression levels increase notably when nematodes are exposed to 2-AG. This observation is supported by fluorescence microscopy images showing elevated GFP signal in ASI neurons treated with 2-AG compared to control groups. Statistical analysis confirms a significant difference in daf-7 expression between the 2-AG treatment and solvent control conditions. The study conducted three independent experiments, analyzing a total of 70 ASI neurons for each condition. Regarding the methods, worms were cultured on solid NGM plates supplemented with bacteria and specific supplements for each experimental condition. Daf-7 expression was monitored in synchronized L1 larvae fed with Escherichia coli supplemented with either 2-AG or carrier solvent. Fluorescence microscopy was utilized to visualize GFP intensity in ASI neurons, and image analysis software quantified the data. The study concludes a robust increase in daf-7 expression in response to 2-AG treatment. | |
| dc.description.fil | Fil: Galles, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratorio de Fisiología Microbiana; Argentina. | |
| dc.description.fil | Fil: Prez, Gastón M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratorio de Fisiología Microbiana; Argentina. | |
| dc.description.fil | Fil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratorio de Fisiología Microbiana; Argentina. | |
| dc.description.sponsorship | Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2155, PICT 3693 | |
| dc.description.sponsorship | Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) | |
| dc.description.sponsorship | Fundación Bunge y Born | |
| dc.description.version | peerreviewed | |
| dc.format.extent | 1-2 | |
| dc.identifier.issn | 2578-9430 | |
| dc.identifier.uri | https://hdl.handle.net/2133/26938 | |
| dc.language.iso | en | |
| dc.publisher | Caltech Library | |
| dc.relation.publisherversion | https://doi.org/10.17912/micropub.biology.000056 | |
| dc.relation.publisherversion | https://www.micropublication.org/journals/biology/micropub-biology-000056 | |
| dc.rights | openAccess | |
| dc.rights.holder | Galles, Celina | |
| dc.rights.holder | Prez, Gastón M. | |
| dc.rights.holder | De Mendoza, Diego | |
| dc.rights.holder | Universidad Nacional de Rosario | |
| dc.rights.text | Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | 2-arachidonoyl glycerol | |
| dc.subject | Daf-7 | |
| dc.subject | ASI neurons | |
| dc.subject | Nematodes | |
| dc.subject | Strain FK181 ksIs2 | |
| dc.subject | Acetonitrile | |
| dc.title | Endocannabinoid 2-arachidonoyl glycerol increases the transcription of daf-7 in ASI neurons | |
| dc.type | articulo | |
| dc.type.collection | articulo | |
| dc.type.version | publishedVersion |