Hepatic preneoplasia induction in male Wistar rats: histological studies up to five months post treatment
| dc.citation.title | Revista Española de Enfermedades Digestivas (REED) | |
| dc.citation.volume | 108 | |
| dc.creator | Pisani, Gerardo Bruno | |
| dc.creator | Valenti, José Luis | |
| dc.creator | Quintana, Alejandra Beatriz | |
| dc.date.accessioned | 2024-07-03T13:29:40Z | |
| dc.date.available | 2024-07-03T13:29:40Z | |
| dc.date.issued | 2016-08 | |
| dc.description.abstract | Background: Liver preneoplasia development in rats can be mimicked by an initiation-promotion model that induces the appearance of altered hepatocyte foc (FAH). Aims: We compare two initiation-promotion models to evaluate the presence of FAH or additional hepatic pathologies in which other organs were affected up to five month post treatment. Material and methods: FAH were induced in male adult Wistar rats with two doses of dietylnitrosamine (DEN, 150 mg/kg bw) followed by 4 doses per week (3 weeks) of 2-acetylaminofluorene (2-AAF, 20 mg/kg bw) or with one dose of DEN (200 mg/kg bw) followed by 2 doses per week (3 weeks) of 2-AAF. DEN 150, DEN 200 and control mice (received the vehicle of the drugs) groups were compared. Rats were euthanized immediately after the last dose of 2-AAF, at 3, 4 and 5 months (n = 3 euthanasia times per group). Samples of livers, lungs, kidneys, pancreatic tissue and small bowel were processed for histological and immunohistochemical analysis. Results: FAH persisted for 5 months in all livers of the DEN groups. Three months after withdrawal of 2-AAF, one rat from the DEN 150 group developed fibrosis and 5 months after 2-AAF removal another rat from the same group presented a microscopic hyperplastic nodule. Only the lungs had damage compatible with lesions induced by gavage-related reflux in DEN groups. Conclusion: We concluded that up to five month post treatments, FAH persisted in all the livers from the DEN groups; livers from the DEN 200 group showed no other hepatic lesions besides FAH, and only the lungs suffered pathological alterations in both treated groups. | |
| dc.description.fil | Fil: Pisani, Gerardo Bruno. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Fisiología. Área de Morfología; Argentina. | |
| dc.description.fil | Fil: Quintana, Alejandra Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Fisiología. Área de Morfología; Argentina. | |
| dc.description.fil | Fil: Valenti, José Luis. Universidad Abierta Interamericana. Facultad de Medicina y Ciencias de la Salud. Área de Patología; Argentina. | |
| dc.description.sponsorship | Secretaría de Ciencia, Tecnología e Innovación de la provincia de Santa Fe (SeCTeI): Project No. 2010-093-11 | |
| dc.description.version | peerreviewed | |
| dc.format.extent | 1-7 | |
| dc.identifier.issn | 1130-0108 | |
| dc.identifier.uri | https://hdl.handle.net/2133/27404 | |
| dc.language.iso | en | |
| dc.publisher | Sociedad Española de Patología Digestiva (SEPD) | |
| dc.relation.publisherversion | https://doi.org/10.17235/reed.2016.4183/2015 | |
| dc.relation.publisherversion | https://www.reed.es/hepatic-preneoplasia-induction-in-male-wistar-rats-histological-studies-up-to-five-months-post-treatment1057 | |
| dc.rights | openAccess | |
| dc.rights.holder | Pisani, Gerardo Bruno | |
| dc.rights.holder | Valenti, José Luis | |
| dc.rights.holder | Quintana, Alejandra Beatriz | |
| dc.rights.holder | Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas | |
| dc.rights.text | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Diethilnitrosamine | |
| dc.subject | 2-acetylaminofluorene | |
| dc.subject | Rat liver preneoplasia | |
| dc.subject | Foci of altered hepatocytes | |
| dc.subject | Reflux | |
| dc.title | Hepatic preneoplasia induction in male Wistar rats: histological studies up to five months post treatment | |
| dc.type | articulo | |
| dc.type.collection | articulo | |
| dc.type.version | publishedVersion |