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Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity

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dc.citation.titleJournal of Environmental Pathology, Toxicology and Oncologyes
dc.citation.volume33(2)es
dc.creatorCapitaine Funes, Juan
dc.creatorMassa, Estefania
dc.creatorCipulli, Germán
dc.creatorBenitez Gil, Alfonso
dc.creatorCapitaine Funes, Carlos
dc.creatorTozzini, Roberto
dc.creatorGhersevich, Sergio
dc.creatorCeballos Mancini, María Paula
dc.date.accessioned2017-11-23T18:41:19Z
dc.date.available2017-11-23T18:41:19Z
dc.date.issued2014-04-15
dc.descriptionIt is accepted that radiotherapy and chemotherapy cause genotoxic effects as part of their therapy side effects, which are highly variable among different patients. This study evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before (“pre-treatment patients”; n = 47) and after (“post-treatment patients”; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0-4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analysis. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. Results showed a significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001). The % head DNA in post-treatment samples from patients with high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patient with low to medium level of DNA damage before therapy (49.2 ± 4.4%). Results support the usefulness of the comet assay as a sensitive technique to evaluate the basal DNA status and the damage caused by cancer treatments. The assay could contribute to the treatment decisions, taking into account the patients’ basal DNA damage before therapy.es
dc.description.filFil: Capitaine Funes, Juan. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentinaes
dc.description.filFil: Ceballos, María Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (UNR-CONICET); Argentinaes
dc.description.filFil: Capitaine Funes, Juan. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentinaes
dc.description.filFil: Massa, Estefania. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes
dc.description.filFil: Cipulli, Germán. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentinaes
dc.description.filFil: Benitez Gil, Alfonso. Provincia de Santa Fe. Hospital Provincial. Servicio de Mastología; Argentinaes
dc.description.filFil: Capitaine Funes, Carlos. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentinaes
dc.description.filFil: Capitaine Funes, Carlos. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentinaes
dc.description.filFil: Tozzini, Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentinaes
dc.description.filFil: Tozzini, Roberto. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentinaes
dc.description.filFil: Ghersevich, Sergio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentinaes
dc.formatapplication/pdf
dc.format.extent111-121es
dc.identifier.issn0731-8898es
dc.identifier.urihttp://hdl.handle.net/2133/9378
dc.language.isoenges
dc.publisherBEGELL HOUSE INCes
dc.relation.publisherversionhttp://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,249005dc454eb8de,570be1034750e71e.htmles
dc.relation.publisherversionDOI: 10.1615/JEnvironPatholToxicolOncol.2014010592es
dc.rightsopenAccesses
dc.rights.holderBegell House Inc.es
dc.rights.holderCeballos Mancini, María Paulaes
dc.rights.holderCapitaine Funes, Juanes
dc.rights.holderMassa, Estefaníaes
dc.rights.holderCipulli, Germánes
dc.rights.holderBenitez Gil, Alfonsoes
dc.rights.holderCapitaine Funes, Carloses
dc.rights.holderTozzini, Robertoes
dc.rights.holderGhersevich, Sergioes
dc.subjectEnsayo cometaes
dc.subjectDaño genotóxicoes
dc.subjectCáncer mamases
dc.subjectFactor pronósticoes
dc.subjectPeripheral blood lymphocyteses
dc.subjectComet assayes
dc.subjectGenotoxic damagees
dc.titleRelationship between genotoxic effects of breast cancer treatments and patient basal DNA integrityes
dc.typearticle
dc.typeartículo
dc.typeacceptedVersion
dc.type.collectionarticulo
dc.type.versionacceptedVersiones

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