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Interference of HTLV-1 Tax Protein with Cell Polarity Regulators: Defining the Subcellular Localization of the Tax-DLG1 Interaction

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dc.citation.titleViruses
dc.citation.volume9 (12)
dc.creatorMarziali, Federico Emanuel
dc.creatorBugnon Valdano, Marina Paula
dc.creatorBrunet Avalos, Clarise
dc.creatorMoriena, Lucía
dc.creatorCavatorta, Ana Laura
dc.creatorGardiol, Daniela
dc.date.accessioned2018-01-22T23:30:32Z
dc.date.available2018-01-22T23:30:32Z
dc.date.issued2017-11-23
dc.descriptionHuman T cell leukemia virus (HTLV)-1 Tax (Tax) protein is very important in viral replication and cell transformation. Tax localizes in the nucleus and cytoplasm in association with organelles. Some activities of Tax depend on interactions with PDZ (PSD-95/Discs Large/Z0-1) domain–containing proteins such as Discs large protein 1 (DLG1) which is involved in cell polarity and proliferation. The DLG1 interaction results in a cytoplasmic co-localization pattern resembling vesicular aggregates, the nature of which is still unknown. To further explore the role of PDZ proteins in HTLV-1 cell transformation, we deeply investigated the Tax-DLG1 association. By fluorescence resonance energy transfer (FRET), we detected, for the first time, the direct binding of Tax to DLG1 within the cell. We showed that the interaction specifically affects the cellular distribution of not only DLG1, but also Tax. After studying different cell structures, we demonstrated that the aggregates distribute into the Golgi apparatus in spatial association with the microtubule-organizing center (MTOC). This study contributes to understand the biological significance of Tax-PDZ interactionses
dc.description.filFil: Marziali, Federico Emanuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina.
dc.description.filFil: Bugnon Valdano, Marina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina.
dc.description.filFil: Brunet Avalos, Clarise. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina.
dc.description.filFil: Moriena, Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina.
dc.description.filFil: Cavatorta, Ana Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina.
dc.description.filFil: Gardiol, Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina.
dc.description.sponsorshipFundación Bunge y Born for the Oster Fellowship to FM.
dc.description.sponsorshipThis work was partially supported by a grant from the Instituto Nacional del Cáncer, Ministerio de Salud de la República Argentina to DG.
dc.formatapplication/pdf
dc.format.extent355, 1-19
dc.identifier.issn1999-4915
dc.identifier.urihttp://hdl.handle.net/2133/10468
dc.language.isoenges
dc.publisherMDPIes
dc.relation.publisherversionhttp://dx.doi.or/doi:10.3390/v9120355es
dc.relation.publisherversionhttp://www.mdpi.com/1999-4915/9/12/355es
dc.rightsopenAccesses
dc.rights.holderMarziali, Federico Emanueles
dc.rights.holderBugnon Valdano, Marina Paulaes
dc.rights.holderBrunet Avalos, Clarisees
dc.rights.holderMoriena, Lucíaes
dc.rights.holderCavatorta, Ana Lauraes
dc.rights.holderGardiol, Danielaes
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/*
dc.subjectHTLV-1es
dc.subjectTaxes
dc.subjectDLG1es
dc.subjectProtein Interactionses
dc.subjectPDZes
dc.titleInterference of HTLV-1 Tax Protein with Cell Polarity Regulators: Defining the Subcellular Localization of the Tax-DLG1 Interactiones

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