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Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers

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dc.citation.titleEuphyticaes
dc.citation.volume124es
dc.creatorCairo, Carlos Alberto
dc.creatorStein, Juliana
dc.creatorDelgado, Luciana
dc.creatorBortolotti, Santiago
dc.creatorGuelman, Sebastián A.
dc.creatorOrtiz, Juan Pablo A.
dc.creatorMorandi, Eligio
dc.date.accessioned2018-12-11T14:00:00Z
dc.date.available2018-12-11T14:00:00Z
dc.date.issued2002-07-20
dc.descriptionSoybean cultivars carrying the ‘long juvenile trait’ show a delayed flowering response under short day conditions. The incorporation of this character into genotypes of agronomic interest may allow a broader range of sowing dates and latitudes for a single cultivar adaptation. The objective of this work was to identify molecular markers linked to the juvenile locus in soybean. Experiments were carried out using two pairs of near isogenic lines (NILs) differing in the presence of the long juvenile trait, and RAPD markers. Four hundred primers were first screened to find polymorphism associated with the trait. Additional differences between NILs were sought by digesting the genomic DNA with five restriction enzymes. Polymorphic fragments detected between NILs were tested for linkage to the juvenile locus in the corresponding F2 segregating populations. Marker bc357-HaeIII was linked (χ2L = 46.316) to the juvenile locus with an estimated recombination frequency of 0.13 ± 0.03 in one of the genetic backgrounds studied. The fragment was cloned, sequenced and converted into a SCAR marker. Moreover, bc357-HaeIII was used as RFLP probe. Both, SCAR and RFLP generated markers linked to the juvenile locus in the two genetic backgrounds analysed. Results presented in this work can be utilised for both, the localisation of the gene associated with the character and for tagging the juvenile trait in soybean breeding programs.es
dc.description.filLaboratorio de Fisiolog´ıa Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, CC 14 (2123) Zavalla, Provincia de Santa Fe, Argentinaes
dc.formatapplication/pdf
dc.format.extent387–395es
dc.identifier.issn0014-2336es
dc.identifier.urihttp://hdl.handle.net/2133/13774
dc.language.isoenges
dc.publisherKluwer Academic Publisherses
dc.relation.publisherversionhttps://link.springer.com/article/10.1023/A:1015767623038es
dc.rightsopenAccesses
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectGlycine maxes
dc.subjectjuvenile locuses
dc.subjectmolecular markerses
dc.subjectRAPDes
dc.titleTagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markerses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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