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Whole genome re-sequencing analysis of two tomato genotypes for polymorphism insight in cloned genes and a genetic map construction

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dc.citation.titleScientia Horticulturaees
dc.citation.volume247es
dc.creatorCambiaso, Vladimir
dc.creatorPratta, Guillermo Raúl
dc.creatorPereira da Costa, Javier Hernán
dc.creatorZorzoli, Roxana
dc.creatorFrancis, David Merrel
dc.creatorRodríguez, Gustavo Rubén
dc.date.accessioned2019-03-21T18:12:50Z
dc.date.available2019-03-21T18:12:50Z
dc.date.issued2019
dc.descriptionNext generation sequencing technologies have become affordable for most plant breeding programs. In this study we sequenced the entire genome of the Solanum lycopersicum L. cultivar Caimanta and S. pimpinellifolium L. accession LA0722 with assembly relative to the Heinz 1706 reference version SL2.50. We present 1) analysis of the amount and distribution of polymorphism in “Caimanta” and “LA0722”, 2) examination of alleles in candidate genes affecting disease resistance, fruit shape, fruit weight and fruit quality and 3) development of molecular markers to construct a genetic linkage map based on a F2 population. A total of 1,397,518 polymorphisms were detected in the comparison between “Caimanta” and “LA0722”. A resistant allele for Rx4/Xv3 was detected by sequence, and confirmed through inoculation. We developed a set of insertion/deletion (InDel) DNA markers that can be multiplexed and scored using easily accessed genotyping platforms. These markers were used to construct a genetic linkage map. We demonstrate that the whole genome sequencing of parental lines can be successfully used to reveal phenotypes and characterize a reference population through easily accessed genotyping strategies.es
dc.description.filFil: Cambiaso, Vladimir. Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET-UNR). Campo Experimental Villarino, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, S2125ZAA Zavalla, Argentina.es
dc.description.filFil: Zorzoli, Roxana. Consejo de Investigaciones de la Universidad Nacional de Rosario. Cátedra de Genética, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, S2125ZAA Zavalla, Argentina.es
dc.description.filFil: Francis, David Merrel. Department of Horticulture and Crop Sciences, The Ohio State University, OARDC, 1680 Madison Ave. Wooster, OH 44691, USA.es
dc.description.sponsorshipFunding for this research has been provided by Agencia Nacional de Promoción Científica y Tecnológica PICT2015-0424, Consejo Nacional de Investigaciones Científicas y Técnicas PIP008, and Universidad Nacional de Rosario PID UNR AGR 247 to GRR.es
dc.formatapplication/pdf
dc.format.extent58-66es
dc.identifier.issn0304-4238es
dc.identifier.urihttp://hdl.handle.net/2133/14269
dc.language.isoenges
dc.publisherElsevieres
dc.relation.publisherversionhttps://doi.org/10.1016/j.scienta.2018.12.001es
dc.rightsopenAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/*
dc.subjectInDel markerses
dc.subjectLinkage mapes
dc.subjectNext generation sequencinges
dc.subjectSolanum spp.es
dc.subjectVariant callinges
dc.titleWhole genome re-sequencing analysis of two tomato genotypes for polymorphism insight in cloned genes and a genetic map constructiones
dc.typearticle
dc.typeartículo
dc.typeacceptedVersion
dc.type.collectionarticulo
dc.type.versionacceptedVersiones

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