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Identification of FadAB complexes involved in fatty acid β-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing strain

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dc.citation.titleFrontiers in Microbiologyes
dc.citation.volume8es
dc.creatorMenendez Bravo, Simón M.
dc.creatorPaganini, Julián
dc.creatorAvignone-Rossa, Claudio
dc.creatorGramajo, Hugo Cesar
dc.creatorArabolaza, Ana Lorena
dc.date.accessioned2021-03-10T15:49:59Z
dc.date.available2021-03-10T15:49:59Z
dc.date.issued2017-08-02
dc.descriptionOleaginous microorganisms represent possible platforms for the sustainable production of oleochemicals and biofuels due to their metabolic robustness and the possibility to be engineered. Streptomyces coelicolor is among the narrow group of prokaryotes capable of accumulating triacylglycerol (TAG) as carbon and energy reserve. Although the pathways for TAG biosynthesis in this organism have been widely addressed, the set of genes required for their breakdown have remained elusive so far. Here, we identified and characterized three gene clusters involved in the β-oxidation of fatty acids (FA). The role of each of the three different S. coelicolor FadAB proteins in FA catabolism was confirmed by complementation of an Escherichia coli1fadBA mutant strain deficient in β-oxidation. In S. coelicolor, the expression profile of the three gene clusters showed variation related with the stage of growth and the presence of FA in media. Flux balance analyses using a corrected version of the current S. coelicolor metabolic model containing detailed TAG biosynthesis reactions suggested the relevance of the identified fadAB genes in the accumulation of TAG. Thus, through the construction and analysis of fadAB knockout mutant strains, we obtained an S. coelicolor mutant that showed a 4.3-fold increase in the TAG content compared to the wild type strain grown under the same culture conditions.es
dc.descriptionPara citar este articulo: Menendez-Bravo S, Paganini J, Avignone-Rossa C, Gramajo H and Arabolaza A (2017) Identification of FadAB Complexes Involved in Fatty Acid β-Oxidation in Streptomyces coelicolor and Construction of a Triacylglycerol Overproducing strain. Front. Microbiol. 8:1428. doi: 10.3389/fmicb.2017.01428
dc.description.filFil: Menendez Bravo, Simón M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Menendez Bravo, Simón M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.es
dc.description.filFil: Paganini, Julián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Paganini, Julián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.es
dc.description.filFil: Avignone-Rossa, Claudio. University of Surrey. School of Biosciences and Medicine. Department of Microbial Sciences; United Kingdom.es
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.es
dc.description.filFil: Arabolaza, Ana Lorena. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Arabolaza, Ana Lorena. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2011-2005es
dc.description.sponsorshipUK Biotechnology and Biological Sciences Research Council: Grant BB/L02683X/1es
dc.formatapplication/pdf
dc.format.extent1-17es
dc.identifier.issn1664-302Xes
dc.identifier.urihttp://hdl.handle.net/2133/20102
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2017.01428es
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2017.01428/fulles
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderMenendez Bravo, Simón M.es
dc.rights.holderPaganini, Juliánes
dc.rights.holderAvignone-Rossa, Claudioes
dc.rights.holderGramajo, Hugo Cesares
dc.rights.holderArabolaza, Ana Lorenaes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectStreptomyces coelicolores
dc.subjectTriacylglycerol Accumulationes
dc.subjectChemostat Systemes
dc.subjectBeta Oxidation of Fatty Acidses
dc.titleIdentification of FadAB complexes involved in fatty acid β-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing straines
dc.titleIdentification of FadAB complexes involved in fatty acid beta-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing straines
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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