Supplementary Materials - Antitumor activity of new chemical compounds in triple negative mammary adenocarcinoma models

Aims. According to the need for the development of new anticancer agents, we have synthetized novel bioactive compounds and aimed to determine their antitumor action. Materials & Methods. We describe in vitro studies evaluating the effect of 35 novel chemical compounds on two triple negative murine mammary adenocarcinoma tumors. Results & Conclusions. Three compounds were selected because of their high antitumor activity and their low toxicity to normal cells. Their effect on tumor cells apoptosis, clonogenicity and migratory capacity, were determined. We found that the selected compounds showed inhibition of viability and clonogenic capacity, and promotion of apoptosis. They also decreased the migratory capacity of tumor cells. The results obtained suggest the likelihood of their future use as antitumor and/or antimetastatic agents SM1: Methodology and characterization of the chemical compounds SM2: Chemical structure of the compounds belonging to each family SM3: Effect of the compounds (100 µM) on 4T1 cells viability. Cells were incubated for 36 hs in complete medium with 100 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM4: Effect of the compounds (75 µM) on 4T1 cells viability. Cells were incubated for 36 hs in complete medium with 75 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM5: Effect of the compounds (50 and 25 µM) on 4T1 cells viability. A) Cells were incubated for 36 hs in complete medium with 50 µM of each compound; B) Cells were incubated for 36 hs in complete medium with 25 µM of each compound. Viable cell number was evaluated with WST-1. Results are shown as percentage of cell viability relative to the non-treated control (100%) and expressed as mean ± SEM. Experiments were performed in triplicate. SM6: The percent of viability of the selected compounds at 25 µM