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The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters

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dc.citation.titleActa Tropicaes
dc.citation.volume120(3)es
dc.creatorCupello Peixoto, Mauricio
dc.creatorFernandes de Souza, Cíntia
dc.creatorBuchensky, Celeste
dc.creatorRocha Corrêa Soares, Juliana Baptista
dc.creatorTravassos Laranja, Gustavo Augusto
dc.creatorGarcia Pinto Coelho, Marsen
dc.creatorCricco, Julia Alejandra
dc.creatorPaes, Marcia Cristina
dc.date.accessioned2020-06-16T15:07:21Z
dc.date.available2020-06-16T15:07:21Z
dc.date.issued2011
dc.descriptionHeme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutritional needs because they do not synthesize this cofactor. However, the mechanisms involved in heme transport across biological membranes are poorly understood. Indeed, in T. cruzi, no heme transporter has yet been characterized. In the present work, we evaluate the heme uptake processes by T. cruzi epimastigotes using fluorescent heme-analogues. Heme uptake decreased significantly when cells were pretreated with different concentrations of SnPPIX, PdMPIX or ZnMPIX, this observed competition suggests that they are taken up by the same transport system. We studied the growth behavior of epimastigotes using the same heme-analogues and the treatments with SnPPIX or PdMPIX impaired cell growth but when heme was added to the culture medium the observed inhibition was partially reversed. In addition, we tested how the heme uptake processes are affected by the presence of different transporter inhibitors. When the cells were treated with inhibitors and then incubated with heme, heme uptake decreased significantly for all treatments. These results constitute a strong indication for the existence of a protein associated with porphyrin transport in T. cruzi, possibly ATP-binding cassette transporters (ABC-transporter).es
dc.description.filFil: Cupello Peixoto, Mauricio. Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes (IBRAG). Departamento de Bioquímica. Laboratório de Interação Tripanossomatídeos e Vetores; Brazil.es
dc.description.filFil: Fernandes de Souza, Cíntia. Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes (IBRAG). Departamento de Bioquímica. Laboratório de Interação Tripanossomatídeos e Vetores; Brazil.es
dc.description.filFil: Buchensky, Celeste. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Rocha Corrêa Soares, Juliana Baptista. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica Redox; Braziles
dc.description.filFil: Travassos Laranja, Gustavo Augusto. Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes (IBRAG). Departamento de Bioquímica. Laboratório de Interação Tripanossomatídeos e Vetores; Brazil.es
dc.description.filFil: Garcia Pinto Coelho, Marsen. Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes (IBRAG). Departamento de Bioquímica. Laboratório de Interação Tripanossomatídeos e Vetores; Brazil.es
dc.description.filFil: Cricco, Julia Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Paes, Marcia Cristina. Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes (IBRAG). Departamento de Bioquímica. Laboratório de Interação Tripanossomatídeos e Vetores; Brazil.es
dc.description.filFil: Paes, Marcia Cristina. Instituto Nacional de Ciência e Tecnologia. Entomologia Molecular (INCT-EM); Braziles
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)es
dc.description.sponsorshipFundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)es
dc.description.sponsorshipInstituto Nacional de Ciência e Tecnologia - Entomologia Molecular (INCT-EM)es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 2010-0685es
dc.description.sponsorshipFundación Bunge y Born: subsidio 2010/2012 para Investigación de la Enfermedad de Chagas (FBB27/10)es
dc.formatapplication/pdf
dc.format.extent211-218es
dc.identifier.issn1873-6254es
dc.identifier.urihttp://hdl.handle.net/2133/18347
dc.language.isoenges
dc.publisherElsevieres
dc.relation.publisherversionhttps://doi.org/10.1016/j.actatropica.2011.08.011es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0001706X11002531?via%3Dihub#!es
dc.rightsopenAccesses
dc.rights.holderElsevieres
dc.rights.holderCupello Peixoto, Mauricioes
dc.rights.holderFernandes de Souza, Cíntiaes
dc.rights.holderBuchensky, Celestees
dc.rights.holderRocha Corrêa Soares, Juliana Baptistaes
dc.rights.holderTravassos Laranja, Gustavo Augustoes
dc.rights.holderGarcia Pinto Coelho, Marsenes
dc.rights.holderCricco, Julia Alejandraes
dc.rights.holderPaes, Marcia Cristinaes
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0*
dc.subjectHemees
dc.subjectTrypanosoma cruzies
dc.subjectPorphyrinses
dc.subjectHeme transportes
dc.subjectBiological Transportes
dc.titleThe heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporterses
dc.typearticle
dc.typeartículo
dc.typeacceptedVersion
dc.type.collectionarticulo
dc.type.versionacceptedVersiones

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