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Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum

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dc.citation.titleFrontiers in Microbiologyes
dc.creatorHabibi Arejan, Neda
dc.creatorEnsinck, Delfina
dc.creatorDiacovich, Lautaro
dc.creatorPatel, Parthvi Bharatkumar
dc.creatorQuintanilla, Samantha Y.
dc.creatorEmami Saleh, Arash
dc.creatorGramajo, Hugo Cesar
dc.creatorBoutte, Cara C.
dc.date.accessioned2023-02-10T12:42:53Z
dc.date.available2023-02-10T12:42:53Z
dc.date.issued2023-01-12
dc.descriptionMycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function.es
dc.descriptionHabibi Arejan N, Ensinck D, Diacovich L, Patel PB, Quintanilla SY, Emami Saleh A, Gramajo H and Boutte CC (2023) Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum. Front. Microbiol. 13:1085918. doi: 10.3389/fmicb.2022.1085918
dc.description.filFil: Habibi Arejan, Neda. University of Texas at Arlington. Department of Biology; United States.es
dc.description.filFil: Ensinck, Delfina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratory of Physiology and Genetics of Actinomycetes; Argentina.es
dc.description.filFil: Diacovich, Lautaro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratory of Physiology and Genetics of Actinomycetes; Argentina.es
dc.description.filFil: Patel, Parthvi Bharatkumar. University of Texas at Arlington. Department of Biology; United States.es
dc.description.filFil: Quintanilla, Samantha Y. University of Texas at Arlington. Department of Biology; United States.es
dc.description.filFil: Boutte, Cara C. University of Texas at Arlington. Department of Biology; United States.es
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratory of Physiology and Genetics of Actinomycetes; Argentina.es
dc.description.filFil: Emami Saleh, Arash. University of Texas at Arlington. Department of Civil Engineering; United States.es
dc.description.sponsorshipNational Institutes of Health (NIH): R01AI148917
dc.formatapplication/pdf
dc.format.extent1-15es
dc.identifier.issn1664-302Xes
dc.identifier.urihttp://hdl.handle.net/2133/25157
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2022.1085918
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2022.1085918/full
dc.rightsopenAccesses
dc.rights.holderHabibi Arejan, Nedaes
dc.rights.holderEnsinck, Delfinaes
dc.rights.holderDiacovich, Lautaroes
dc.rights.holderPatel, Parthvi Bharatkumares
dc.rights.holderQuintanilla, Samantha Y.es
dc.rights.holderEmami Saleh, Arashes
dc.rights.holderGramajo, Hugo Cesares
dc.rights.holderBoutte, Cara C.es
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 International (CC BY 4.0)(CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectACCase complexes
dc.subjectDivIVAes
dc.subjectIntracellular membrane domain (IMD)es
dc.subjectFtsIes
dc.subjectMurGes
dc.subjectMycobacteriumes
dc.subjectPetidoglycan synthesises
dc.titlePolar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septumes
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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