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A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers

dc.citation.titleMolecular Breedinges
dc.citation.volume20es
dc.creatorStein, Juliana
dc.creatorPessino, Silvina Claudia
dc.creatorMartínez, Eric Javier
dc.creatorRodríguez, María Pía
dc.creatorSiena, Lorena Adelina
dc.creatorQuarin, Camilo Luis
dc.creatorOrtiz, Juan Pablo Amelio
dc.date.accessioned2018-12-11T16:04:07Z
dc.date.available2018-12-11T16:04:07Z
dc.date.issued2007-01-29
dc.descriptionPaspalum notatum Flu¨ gge is a warmseason forage grass with mainly diploid (2n = 20) and autotetraploid (2n = 40) representatives. Diploid races reproduce sexually and require crosspollination due to a self-incompatible mating system, while autotetraploids reproduce by aposporous apomixis. The objectives of this work were to develop a genetic linkage map of Paspalum notatum Flu¨gge at the tetraploid level, identify the linkage/s group/s associated with apomixis and carry out a general characterization of its mode of inheritance. A pseudo test-cross F1 family of 113 individuals segregating for the mode of reproduction was obtained by crossing a synthetic completely sexual tetraploid plant (Q4188) as female parent with a natural aposporous individual (Q4117) as pollen donor. Map construction was based on single-dose markers (SDAFs) segregating from both parents. Two linkage maps (female and male) were constructed. Within each map, homologous groups were assembled by detecting repulsion-phase linked SDAFs. Putative Q4188 and Q4117 homolog groups were identified by mapping shared single dose markers (BSDF). The Q4188 map consisted of 263 markers distributed on 26 co-segregation groups over a total genetic distance of 1.590.6 cM, while the Q4117 map contained 216 loci dispersed on 39 co-segregation groups along 2.265.7 cM, giving an estimated genome coverage of 88% and 83%, respectively. Seven and 12 putative homologous chromosomes were detected within Q4188 and Q4117 maps, respectively. Afterward, ten female and male homologous chromosomes were identified by mapping BSDFs. In the Q4117 map, a single linkage group was associated with apospory. It was characterized by restriction in recombination and preferential chromosome pairing. A BPSD marker mapping within this group allowed the detection of the female homolog and the putative four male groups of the set carrying apospory.es
dc.description.filFil: Stein, Juliana. Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario; Argentina.es
dc.formatapplication/pdf
dc.format.extent153-166es
dc.identifier.issn1380-3743es
dc.identifier.urihttp://hdl.handle.net/2133/13791
dc.language.isoenges
dc.publisherSpringer Science+Business Media B.V.es
dc.rightsopenAccesses
dc.rights.holderSpringeres
dc.subjectAposporyes
dc.subjectMapping in autopolyploidses
dc.subjectPaspalum notatumes
dc.subjectSingle dose markerses
dc.titleA genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markerses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.typeMaterial Didáctico
dc.type.collectionarticulo
dc.type.versionpublishedVersiones
lom.educational.contextSuperior no universitarioes
lom.educational.contextPosgradoes
lom.educational.contextONGses
lom.educational.contextCentro CyTes
lom.educational.contextOtroses
lom.educational.difficultyMediana Dificultades
lom.educational.esMDSI*
lom.educational.interactivitymixtaes
lom.educational.typicalAgeRangeadultoses

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