Examinando por Autor "Diacovich, Lautaro"
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Ítem Acceso Abierto 3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection(Public Library of Science (PLOS), 2018-06-07) Tomassetti, Mauro; Garavaglia, Betiana Soledad; Vranych, Cecilia Verónica; Gottig, Natalia; Ottado, Jorgelina; Gramajo, Hugo Cesar; Diacovich, LautaroÍtem Acceso Abierto Complete genome sequence of the Microbacterium foliorum bacteriophage Garey24(American Society for Microbiology, 2024-02-05) Migueletti, Matías R.; García Rey, Julieta; Micheloni, Josefina; Lomanto , Camila; Martelli, Elisa; Sánchez, Gastón; Colombo, Julián M.; Vallecillo, Luciano M.; Lamagni, Francisco; Giusti, Tomás; Acosta, Fabrina; Villagrán, Franco; Gvozdenovich, Martín; Pricco Frakich, Abril; Pianesi, Tulio; Tulin, Gonzalo; Mascali, Florencia Carla; Petitti, Tomás D.; Torres Manno, Mariano A.; Fusari, Corina M.; Buttigliero, Laura; Giordana, María Florencia; Gramajo, Hugo Cesar; Diacovich, Lautaro; Espariz, Martín; Mussi, María Alejandra; http://orcid.org/0000-0002-3339-0100; http://orcid.org/0000-0002-4090-515X; http://orcid.org/0000-0002-4168-3624In this work, we report the discovery and characterization of Garey24, a bacteriophage that forms medium-size plaques with halo rings isolated from a soil sample in Funes, Argentina. Its 41,522 bp circularly permuted genome contains 63 putative protein-coding genes. Based on gene content similarity, Garey24 was assigned to subcluster EA1.Ítem Acceso Abierto Expression and purification of untagged GlnK proteins from actinobacteria(EXCLI, 2017-06-27) Gerhardt, Edileusa C. M.; Moure, Vivian R.; Souza, Andrey W.; Pedrosa, Fabio O.; Souza, Emanuel M.; Diacovich, Lautaro; Gramajo, Hugo Cesar; Huergo, Luciano F.The PII protein family constitutes one of the most conserved and well distributed family of signal transduction proteins in nature. These proteins play key roles in nitrogen and carbon metabolism. PII function has been well documented in Gram-negative bacteria. However, there are very few reports describing the in vitro properties and function of PII derived from Gram-positive bacteria. Here we present the heterologous expression and efficient purification protocols for untagged PII from three Actinobacteria of medical and biotechnological interest namely: Mycobacterium tuberculosis, Rhodococcus jostii and Streptomyces coelicolor. Circular dichroism and gel filtration analysis supported that the purified proteins are correctly folded. The purification protocol described here will facilitate biochemical studies and help to uncover the biochemical functions of PII proteins in Actinobacteria.Ítem Acceso Abierto KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells(Public Library of Science (PLOS), 2019-01-10) Domizi, Pablo; Malizia, Florencia; Chazarreta-Cifre, Lorena; Diacovich, Lautaro; Banchio, ClaudiaÍtem Acceso Abierto Mycobacterium tuberculosis FasR senses long fatty acyl-CoA through a tunnel and a hydrophobic transmission spine(Springer Nature, 2020-07-24) Lara, María Julia; Diacovich, Lautaro; Trajtenberg, Felipe; Larrieux, Nicole; Malchiodi, Emilio L.; Fernández, Marisa M.; Gago, Gabriela; Gramajo, Hugo Cesar; Buschiazzo, Alejandro; https://orcid.org/0000-0002-3339-0100; https://orcid.org/0000-0003-0427-5549; https://orcid.org/0000-0001-7501-3330; https://orcid.org/0000-0001-7668-0119; https://orcid.org/0000-0002-2509-6526Mycobacterium tuberculosis is a pathogen with a unique cell envelope including very long fatty acids, implicated in bacterial resistance and host immune modulation. FasR is a TetR-like transcriptional activator that plays a central role in sensing mycobacterial long-chain fatty acids and regulating lipid biosynthesis. Here we disclose crystal structures of M. tuberculosis FasR in complex with acyl effector ligands and with DNA, uncovering its molecular sensory and switching mechanisms. A long tunnel traverses the entire effector-binding domain, enabling long fatty acyl effectors to bind. Only when the tunnel is entirely occupied, the protein dimer adopts a rigid configuration with its DNA-binding domains in an open state, leading to DNA dissociation. The protein-folding hydrophobic core connects the two domains, and is completed into a continuous spine when the effector binds. Such a transmission spine is conserved in a large number of TetR-like regulators, offering insight into effector-triggered allosteric functional control.Ítem Acceso Abierto Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum(Frontiers Media, 2023-01-12) Habibi Arejan, Neda; Ensinck, Delfina; Diacovich, Lautaro; Patel, Parthvi Bharatkumar; Quintanilla, Samantha Y.; Emami Saleh, Arash; Gramajo, Hugo Cesar; Boutte, Cara C.