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Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528

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dc.citation.titleBiotechnology Reports
dc.creatorLario, Luciana Daniela
dc.creatorPillaca-Pullo, Omar Santiago
dc.creatorSette, Lara Durães
dc.creatorConverti, Attilio
dc.creatorCasati, Paula
dc.creatorSpampinato, Claudia P.
dc.creatorPessoa, Adalberto
dc.date.accessioned2024-06-06T18:21:51Z
dc.date.available2024-06-06T18:21:51Z
dc.date.issued2020-10-21
dc.description.abstractEnzymes from cold-adapted microorganisms are of high interest to industries due to their high activity at low and mild temperatures, which makes them suitable for their use in several processes that either require a supply of exogenous energy or involve the use of heat labile products. In this work, the protease production by the strain Rhodotorula mucilaginosa CBMAI 1528, previously isolated from the Antarctic continent, was optimized, and the purified enzyme analyzed. It was found that protease production was dependent on culture medium composition and growth temperature, being 20 C and a culture medium containing both glucose and casein peptone (20 and 10 g/L, respectively) the optimal growing conditions in batch as well as in bioreactor. Moreover, mass spectrometry analysis revealed that the enzyme under study has a 100 % sequence identity with the deduced amino acid sequence of a putative aspartic protease from Rhodotorula sp. JG-1b (protein ID: KWU42276.1). This result was confirmed by the decrease of 95 % proteolytic activity by pepstatin A, a specific inhibitor of aspartic proteases. We propose that the enzyme reported here could be Rodothorulapepsin, a protein characterized in 1972 that did not have an associated sequence to date and has been classified as an orphan enzyme.
dc.description.filFil: Lario, Luciana Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
dc.description.filFil: Lario, Luciana Daniela. University of Sao Paulo. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences; Brazil.
dc.description.filFil: Lario, Luciana Daniela. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada (INGEBIO); Argentina.
dc.description.filFil: Pillaca-Pullo, Omar Santiago. University of Sao Paulo. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences; Brazil.
dc.description.filFil: Sette, Lara Durães. Sao Paulo State University. Institute of Biosciences. Department of General and Applied Biology; Brazil.
dc.description.filFil: Converti, Attilio. University of Genoa. Pole of Chemical Engineering. Department of Civil, Chemical and Environmental Engineering; Italy.
dc.description.filFil: Casati, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
dc.description.filFil: Spampinato, Claudia P. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina.
dc.description.filFil: Pessoa, Adalberto. University of Sao Paulo. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences; Brazil.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP): No 2012/23726-4
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT 2017-0356, PICT 2016-2500
dc.format.extent1-9
dc.identifier.issn2215-017X
dc.identifier.urihttps://hdl.handle.net/2133/27238
dc.language.isoen
dc.publisherElsevier
dc.relation.publisherversionhttps://doi.org/10.1016/j.btre.2020.e00546
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2215017X20307773?via%3Dihub
dc.rightsopenAccess
dc.rights.holderLario, Luciana Daniela
dc.rights.holderPillaca-Pullo, Omar Santiago
dc.rights.holderSette, Lara Durães
dc.rights.holderConverti, Attilio
dc.rights.holderCasati, Paula
dc.rights.holderSpampinato, Claudia P.
dc.rights.holderPessoa, Adalberto
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAntarctic yeast
dc.subjectAspartic protease
dc.subjectRodothorulapepsin
dc.titleOptimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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